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KMID : 0860920070090010069
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Journal of the Korean Association EMG-Electrodiagnostic Medicine
2007 Volume.9 No. 1 p.69 ~ p.74
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Myogenic Potential of Human Adipose-Tissue-Derived Cells
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Park Yoon-Ghil
Moon Jae-Ho
Lee Eun-Young
Do Byung-Rok
Kim Gi-Hyang
Kim Goon-Tae
Kim Dong-Soo
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Abstract
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Objectives: Cell therapy has been extensively studied as a gene complementation approach in such genetic diseases as Duchenne muscular dystrophy (DMD). Adipose tissue has recently been identified as an alternative, uniquely abundant and accessible source of pluripotent cells. In the present work we inves- tigated myogenic potentials of adipose-tissue-derived cells (ATDCs) as mesenchymal stem cells (MSCs).
Methods: Human ATDCs were obtained by liposuction and cultured in three different media; control, myogenic and conditioned media. The following observation was made to evaluate differentiation with using immunoflurescence study and western blotting.
Results: Conversion of ATDCs to a myogenic phenotype is observed by indirect immunoflurescence study of MyoD and Myf-5 in regardless of media type. However, secondary myogenic regulatory factors (Myf-6 and myogenin) and desmin are negative in all different culture condition.
Conclusion: Our findings suggest that human adipose-tissue-derived cells might have a mesencymal stem cell population and myogenic potentials. Since human adipose tissue is plentiful, easily harvested in large quantity under local anesthesia with little patient discomfort, it may be a good source of an alterna- tive cell therapy for DMD patients.
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KEYWORD
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Cell therapy, Duchenne muscular dystrophy, Adipose-tissue-derived cells, Mesenchymal
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